MEETING AGENDA

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If you'd prefer a preview - here is the program book.

FRIDAY, September 29th

10 am to 4 pm: CORE MANAGERS WORKSHOP

Co-sponsored by ISAC and GLIIFCA, this annual workshop is open to all involved or interested in lab management. If you have a topic you wish discussed, please email the conveners to volunteer.

6 pm to 11 pm: OPENING RECEPTION

7 pm to 9 pm: INDUSTRIAL SCIENCE SYMPOSIUM

Conveners: A. Nakeff & B. Pesch

1. David Cheo, BD Biosciences: High Content Imaging: Taking Cell Analysis to a Higher Resolution
2. Ruud Hulspas, Cytonome: The Next Phase in Clinical-grade, High-speed Cell Sorting: Parallel Microfluidics Optical Sorters on a Chip
3. David Basiji, Amnis Corp: Thinking Outside the Dot: Quantitative Cell Image Analysis in Flow with the ImageStream System
4. Maria Daly, Beckman-Coulter:. Automation of bead-based flow cytometric analysis to measure 10-plex cytokine arrays in a 96-well plate format

SATURDAY, September 30th

8 am - 10:45 am: CLINICAL INNOVATIONS

Conveners: K. Domenico and P. Wallace

1. Thomas Shanahan: The Flow Cytometry Approach to Transplant Compatibility
2. Raymond Felgar: Application to the WHO Classification of Hematologic Malignancy
3. George Deeb: Flow Cytometry and Clinopathological Correlations: Case Studies

11:00 am - 12:00 pm : THE CARLETON and SIGRID STEWART PLENARY LECTURE

Alan Waggoner: Fluorescent Probes and Imaging Live Cells

12:00 pm - 2:00 pm: LUNCHEON ROUNDTABLES

Conveners: M. Paniagua and J. Auger

Roundtable Discussions:

The Roundtable sessions serve as informal discussion groups conducted over lunch. The primary aim of these discussions is to provide a forum for participants to address their interests in a variety of cytometry and image topics.

1. Imaging Flow Cytometry: Visualizing flow-based assays of immunity Chair: Albert Donnenberg, University of Pittsburgh
2. Laser excitation sources for flow and image cytometry: Updates on the latest laser technology and the implications of their availability for instrumentation upgrades and assay implementation. Chair: Bill Telford, National Cancer Institute
3. Leukemia/Lymphoma discussion group: A two-part discussion including update on latest issues relating to flow cytometric immunophenotyping of leukemias and lymphomas as well as clinicopathologic correlation of phenotype with histology Co-chairs: Paul Wallace and George Deeb, Roswell Park Cancer Institute
4. Polychromatic Flow Cytometry—Beyond Immunophenotyping: discussion of the challenges of using a range of indicator dyes (live/dead discrimination, Qdots, autofluorescence) in addition to monoclonal antibodies for multicolor flow cytometry. Chair: Matthew Hanson, University of Wisconsin
5. Transplant compatibility assessment: Overview of flow cytometry applications for the evaluation of clinically significant allo-reactive antibodies in solid organ transplantation. Chair: Tom Shanahan, Upstate New York Transplant Services
6. Cancer Stem Cell Evaluation : Application of polychromatic flow cytometry to the cancer stem cell paradigm Chair: Vera Donnenberg, University of Pittsburgh
7. Working with Digital Flow Cytometry Data: discussion of digital error, its effect on compensation, and the requirement for Biexponetial/Hyperlog displays Chair: Mark Munson, Verity Software House
8. Do-it-Yourself Service: Focus will be alternatives to traditional service contracts for cytometry instrumentation, i.e. self-service, third party, parts only—as well as advantages/disadvantages to this approach Co-chairs: Ryan Duggan, University of Chicago and Alex Rodriguez, Case Western Reserve University
9. Clinical Flow Cytometry Basics: Operations and management issues relevant to the clinical flow cytometry laboratory including but not limited to inspections, proficiency testing, instrument selection and evaluation, quality control and common applications. Co-chairs: Karen Domenico and Tom Sawyer, Medical University of Ohio
10. Instrument Performance Validation: We all generate lots of cytometry data from day to day but are we generating data that accurately reflects our samples? Understanding your instruments limits of detection as well as available tools for verifying an instrument's performance will be central to this discussion. Chair: Joanne Lannigan, University of Virginia
11. 25 Years Later--The State of HIV Assays in the Clinical Lab: A discussion of new technologies in CD4 counting Chair: Frank Mandy, Natl. HIV and Retrovirology Laboratories, Public Health Agency of Canada

Convener: V. Donnenberg

1. Simon Watkins: Live Cell Imaging Allows Novel Functional Interconnections between Cells of the Immune System
2. Bob Murphy: Automated Interpretation and Modeling of Subcellular Patterns for Systems Biology

3:45 pm - 5:15 pm: CUTTING EDGE

Convener: J. Moore

1. Avinash Bhandoola: From Stem Cell to T-Cell: One Route or Many?
2. Phil McCoy: Circulating Endothelial Cells: Rare or Well-Done?

5:00 pm - 7:00 pm: POSTER SESSION (Poster size is 4 foot by 3 foot)

8:00 pm - ????: BANQUET - "SUPERHEROS of FLOW"

SUNDAY, October 1st

9 am - 12:00: APPLICATION INNOVATIONS

Convener: T. Bushnell

1. William Telford: Excitation Sources for Flow Cytometry: Covering the Visible Spectrum
2. Timothy Ragan: High Throughput Tissue Cytometry on a Two-Photon Microscope
3. Lori Broderick: TBN

CLOSING REMARKS and AWARDS PRESENTATION

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