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R1 |
Software tools for core facility administrative task: We will review a number of free, low-cost or more expensive software tools that facilitate adminstrative tasks including; instrument booking, usage tracking, service request forms, etc... |
David Leclerc, University of Chicago |
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R2 |
Biosafety: A discussion of current NIH guidelines, individual experiences and future directions |
Hank Pletcher, University of Penn. |
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R3 |
Cell Cycle/DNA: Standard and non-standard applications for mammalian and non-mammalian sample types as well as analysis challenges for histogram results. |
Louis King, MSU
Mark Munson, VSH |
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R4 |
Educating the educated and uneducated in a rapidly evolving field of technologies and applications through a core facility: Flow cytometry is a quickly evolving field where users not only have to keep up with new instrumentation technologies but also expanding applications. It is the job of the core facility to keep up with advances in cytometry and facilitate the dissemination of this information to the scientists that we serve. This discussion will cover methods and challenges in educating researchers on current and emerging instrumentation, applications and technologies in cytometry |
A. Nicole White (CCHMC)
Monica Delay (CCHMC) |
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R5 |
Reagents: An overview of recent reagent releases and usage in a multiparameter world. Many alternatives to existing fluorophore choices have been released and questions from customers always follow. The group will discuss a selection of these situations and share experiences. |
Justin Meyers (Purdue) |
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R6 |
Online Design Tools: Many online design tools have appeared on vendor sites in the last few years. This group will review what has been developed to date, why they have been developed and discuss strategies for employing them with current available instrumentation. |
Nick Ostrout (Treestar) |
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R7 |
Instrumentation: A review of newer instrumentation, their strengths and weaknesses, options and their role(s) in a variety of facility styles. |
Charles Kuszynski (Univ. of Nebraska) |
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R8 |
Clinical Cytometry in a Research Core: Expanding resource responsibilities to lower expenses, use resources more efficiently, boost morale and provide professional development opportunity. |
Dagna Sheerar (Univ. of Wisconsin) |
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R9 |
Emerging Technologies: Mass cytometry, multispectral flow, Raman spectroscopy. What will the next generation of cytometry look like and how will it affect our existing shared research facilities. |
Therese Martin (Univ. of Minnesota) |
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R10 |
Troubleshooting and QC: Use of vendor products and homebrew systems in the assessment of instrument performance and problems. Employing a system which allows the timely collection of specific information to enable problem resolution and quick decision-making. |
Matt Cochran (Univ. of Rochester) |
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R11 |
Informatics approaches for flow cytometry data management and analysis: Advances in cytometry have enabled routine generation of large multi-color flow cytometry experiments designed to measure large number of samples across tens of markers and highlighting the need for novel analysis approaches for identifying "interesting" cell populations and comparing them across samples. In addition, these experiments generate a large amount of raw data, making it difficult to manage, analyze and collaborate withe these experiments. the goal of this roundtable is to discuss approaches to address these challenges of flow analysis and data management. |
Nikesh Kotecha (Cytobank) |