GLIIFCA roundtable lunch workshops • •

Section: Single Cell Biology

Topic 1. Single Cell Omics: Opportunities and Challenges, Albert Donnenberg, University of Pittsburgh

Location: Madison I

Analytical flow cytometry and preparative flow-based cell sorting are key technologies to facilitate single-cell Omics of heterogeneous samples. Although examples abound of single cell proteomics, transcriptomics and genomics, there are limitations to all of these applications. This round-table discussion will examine common pitfalls, including purity of sorted cells and lower limits of transcript detection, that currently limit single-cell Omics, and attempt to identify potential solutions.

Topic 2. Single Cell Sorting for Culture and Genomics, Rachael Sheridan, Director Flow Core Facility, Van Andel Institute, Grand Rapids, Michigan.

Location: Madison I

With the increase in single cell genomic analyses and utility of CRISPR-derived clones, researchers are requiring more single cell sorting. We’ll discuss validation of sorter performance for single drop sorts into plates compatible with downstream genomic analysis or culture to maximize success and minimize sample handling. There will be ample opportunity to share experiences with these sorts and the collaboration required with another core that may or may not be within the institution.

Topic 3. Cell Sorting: Setting Yourself Up for Success, Lauren Nettenstrom, University of Wisconsin Comprehensive Cancer Center Flow Cytometry Laboratory

Location: Madison I

We will discuss the steps you need to take, whether you are a sort operator or a customer, well before you get to the instrument to set yourself up for a successful sort. Topics will include education, biosafety, panel design.

Section: Imaging

Topic 4. Tissue Clearing and Deep Tissue Imaging, Evan Meyer, ORVCA Board

Location: Madison II

Advances in tissue clearing techniques and imaging methodologies have enabled cytometry deeper into a variety of intact tissues including whole animal models. Selecting the optimal methodology for tissue preparation, imaging, and data analysis is essential for effective outcomes. This round-table discussion will focus on the factors that go into deciding what clearing technique is right for the scenario and considerations for how the data will be acquired and analyzed.

Topic 5. Maximizing the potential of your imaging flow cytometer, Orla Maguire,

Location: Madison II

Discussion points to include (we may not get to all!)

Section: Experimental Development

Topic 6. High Parameter Flow Cytometry and Panel Design, Victoria Smith (UNMC)

Location: Gazebo

Fluorochromes, antigen density and co-ecpression, instrument characteriscitc, and knowledge of the biology being studied are vital in panel design. How can we minimize spectral overlap and use resolution impacto to our benefit when planning our experiments? What are some other factors we need to consider when planning our experiments? Good quality high parameter data depends on great panel design.

Topic 7, Flow cytometry and small molecule drug development in Pharma: If flow is so good, how is Pharma using it? T. Vincent Shankey, PhD, COE, Shankey Biotechnology Consulting, St. Petersburg, FL

Location: Gazebo

Discussion of assessment of drug development in Pharma, including new methodologies that incorporate machine learning and other novel concepts.

Section: Immunotherapy and Cancer

Topic 8. CD4 Voltage Titration: a Method to Optimize Cytometer Performance, Derek Jones, Flow Cytometry and Cell Sorting Resource laboratory, University of Pennsylvania

Location: Gazebo

How can we ensure that our cytometers are performing optimally, The discussion will address the prupose and procedure of CD4 voltage titrations, the practicality within a shared resource laboratory setting, the benefits provided to users, and alternative methods used to achieve optimization. Detailed SOPs and lists of of required reagents will be made available.

Topic 9. Clinical Test Validation: Transforming Research Flow to Clinical Testing, Mary Reynaud, Lisa Neumeier, CCHMC

Location: Gazebo

We will review the criteria for validating a flow cytometry test for use in a clinical diagnostic laboratory. We will discuss the requirements of accrediting agencies to demonstrate a test is robust, accurate and precise for use in making diagnoses. We hope to provide researchers with some idea of what goes into making a good diagnostic assay and emphasize the differences from testing performed for research.

Section: Non-Mammalian Cytometry

Topic 10. Beyond Cells: “We Sort all of the Above,” Kathryn Fox, UWCCC Flow Cytometry Laboratory & Christiane Hassel, IUB Flow Cytometry Core Facility

Location: Terrace IV

Sometimes preparing samples for analysis and sorting requires thinking outside the box. We’ll discuss what to consider when working with samples that either aren’t human or mouse in origin, or are tricky to analyze or sort in general.

Section: Core Management

Topic 11. SRL Best Practices: The Role in Rigor and Reproducibility, Joanne Lannigan, Director, Flow Cytometry Core, University of Virginia

Location: Terrace IV

In November 2016 an ISAC Shared Resource Laboratory (SRL) Task Force published a document outlining a set of “best practices” for SRLs or Core Facilities to use as a general guide for achieving and maintaining standards of excellence in the services they provide. Recently there has been a great deal of focus on rigor and reproducibility in science. How will the implementation of best practices support rigor and reproducibility? What role do SRLs have in promoting rigor and reproducibility? How will these efforts ultimately benefit the SRL? Come join the discussion to find out.

Topic 12. SCYM Exam, Jessica Back, Associate Director, Microscopy, Imaging, and Cytometry Resources Core, Karmanos Cancer Institute, Wayne State University.

Location: Terrace IV

The new Specialist in Cytometry Certification (SCYM) was created through a joint effort by ISAC, the International Clinical Cytometry Society (ICCS), and the American Society for Clinical Pathology Board of Certification (ASCP BOC) and was launched in fall of 2017. The SCYM replaces the International Cytometry Certification Exam (ICCE) and the ASCP BOC’s Qualification in Cytometry (QCYM) recognition program. The eligibility requirements for new applicants, credential maintenance, and frequently asked questions about the SCYM will be addressed.

Page updated on 2018-09-18 16:42:54 -0400